Optimized reprogramming of human iPSCs to generate distinct neuronal subtypes
Karim, et al. 2020. bit.bio
A robust source of human iPSC-derived neurons would offer an attractive in vitro model for CNS research and high content drug screens. However, conventional iPSC differentiation protocols are often complex, inconsistent, and difficult to scale. Proprietary gene-targeting strategy, opti-ox™, is able to overcome these problems, enabling highly controlled expression of transcription factors to rapidly reprogram human iPSCs into pure somatic cell types. Using opti-ox™, we have manufactured consistent and homogenous cultures of glutamatergic neurons (>80% VGLUT1/2) and GABAergic neurons (VGAT1, GABA), which show homogenous molecular phenotype at single cell transcriptomics resolution. Reprogramming is highly consistent and synchronised, yielding fully functional neurons in less than 14 days. Our technology opens up novel avenues for the development of in vitro models to support research and healthcare innovations.