ioSkeletal Myocytes

A novel human skeletal muscle in vitro model using opti-ox™ mediated cellular reprogramming of induced pluripotent stem cells

Bernard, et al. 2020. ioSkeletal myocytes poster download

We have developed an optimised inducible system (opti-ox™) that enables tightly controlled expression of transcription factors improving cellular reprogramming approaches for the differentiation of hiPSCs. Through targeting of genomic safe harbour loci, we used opti-ox™ to achieve homogenous, inducible expression of the myogenic regulator MYOD1. MYOD1 induction leads to shutdown of the core pluripotency network, and activation of key myogenic factors including myosin heavy chain. opti-ox™ reprogrammed ioSkeletal Myocytes express Desmin, Dystrophin and Titin, and form contractile, striated and multinucleated myocytes by Day 10 post-revival. Critically for metabolic studies, robust expression of the insulin-regulated glucose transporter GLUT4 is also detected. Importantly, skeletal myocytes produce a highly pure MHC positive population of cells within 4 days of thawing, comparable to day 10 transdifferentiated fibroblasts, and are suitable for phenotypic based HTS.

Download resource

    Please complete the form below.

    Please confirm that we may add you to our mailing list, and send you our latest news and updates:
    Yes, please email me

    You can unsubscribe at any time by clicking the link in the footer of our emails. Privacy policy.

    This site is protected by reCAPTCHA and the Google Privacy Policy and Terms apply.

    Discover more resources