Human-induced skeletal myocytes
EA1200 | Early access product
Introducing highly-defined, consistent and reliable human muscle cells for research, disease modelling and high throughput screening across areas such as muscle, neuromuscular, and associated metabolic disorders. ioSkeletal Myocytes have been reprogrammed from human induced pluripotent stem cells (iPSCs) using our precise reprogramming technology: opti-ox™¹.
The technology allows for human pluripotent stem cells, within days, to convert into skeletal myocytes, providing a high-quality, easy to use, cellular model.
Skeletal myocytes demonstrate robust expression of components of the contractile apparatus and form striated, multinucleated, myocytes by Day 10 post revival, that contract in response to acetylcholine.
Unprecedented batch-to-batch consistency allows for their use in demanding screening applications where small changes need to be detected against background noise.
Batch to batch reproducibility and homogeneity create a stable human model for the study of muscle, neuromuscular, and associated metabolic disorders.
Form striated, multinucleated, myocytes by Day 10 post revival, that contract in response to acetylcholine.
Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.
Cells arrive programmed to rapidly mature upon revival. One medium required in a two-step protocol.
ioSkeletal Myocytes generated by MYOD1-driven reprogramming of iPSCs using opti-ox™ technology
Video capturing the rapid morphological changes from iPSCs upon induction of MYOD1 expression using opti-ox™ cellular reprogramming. 10 day time course.
ioSkeletal Myocytes express skeletal myocyte-specific markers
Cells demonstrate classical myocyte morphology
Cells demonstrate gene expression of key myogenic markers following reprogramming
Cells express the insulin regulated glucose transporter GLUT4, critical for metabolic studies
Cells are suitable for phenotypic based high-throughput screening
(A) Human fibroblasts were transduced with lentiviral vectors allowing inducible over-expression of MYOD1 to transdifferentiate them to myocytes in approximately 10 days. Transdifferentiated myotubes were stained for multiple myotube markers to assess the purity and degree of multi-nucleation. (B) ioSkeletal Myocytes generate myocytes within as little as 4 days post-revival with a high-degree of MHC+ cells (>95% purity), suitable for phenotypic based high throughput screens. (C) Comparable total area of MHC positive cells are generated between ioSkeletal Myocytes and transdifferentiated fibroblasts.
Shushant Jain et al, Charles River Laboratories
In vitro human muscle cells suitable for contractility assays
By Day 10 post-revival, cells demonstrate a strong contractile response upon addition of acetylcholine, providing a suitable human muscle model for contractility assays. Spontaneous contraction is also observed during continuous culture (data not shown). Day 10 post-revival skeletal myocytes; 50µM acetylcholine.
Cells arrive ready to plate
Available in two vial sizes, tailored to suit your experimental needs with minimal waste
Catalogue number: EA1200 Manufacture site: United Kingdom Sizes: Small & Large Product use: These cells are for research use only Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments Shipping info: Dry Ice Note: Limited Use License | Statement of Use