Protocol library

CNS co-culture

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Co-culture of hiPSC-derived ioGlutamatergic Neurons and ioMicroglia

Protocol for culturing human iPSC-derived glutamatergic neurons (ioGlutamatergic Neurons) and microglia cells (ioMicroglia).

CNS tri-culture

Close-up image of several neurons, nuclei stained blue (DAPI) and cell bodies stained red (MAPT)

Tri-culture of ioGlutamatergic Neurons, ioGABAergic Neurons and astrocytes in 48-well plates for functional MEA analysis

Protocol to tri-culture ioGlutamatergic Neurons, ioGABAergic Neurons and astrocytes in 48-well plates for functional micro-electrode array (MEA) analysis using Axion Maestro Pro

Cell culture and preparation

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Cell detachment for flow cytometry and single cell experiments

Protocol for detaching human iPSC-derived microglia cells for flow cytometry and other single cell experiments.

Cell characterisation and assays

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Immunocytochemical staining protocol for human iPSC-derived GABAergic neurons

Immunocytochemical staining of human iPSC-derived GABAergic neurons to identify, characterise, and validate in vitro.

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Immunocytochemical staining protocol for human iPSC-derived glutamatergic neurons

Immunocytochemical staining of human iPSC-derived glutamatergic neurons to identify, characterise, and validate in vitro.

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ioMicroglia stimulation for cytokine release

Protocol describing the functional characterisation of human iPSC-derived microglia cells via stimulation and cytokine release.

Immunocytochemistry imaging of ioOligodendrocyte-like cells for markers O4 (oligodendrocyte specific marker) and MBP (myelin basic protein).

Immunocytochemistry staining protocol for human iPSC-derived oligodendrocyte-like cells

Immunocytochemistry staining of human iPSC-derived oligodendrocyte-like cells with O4 and MBP antibodies.

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Immunocytochemical staining protocol for human iPSC-derived skeletal myocytes

Immunocytochemical staining of human iPSC-derived skeletal myocytes to identify, characterise, and validate in vitro.

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