opti-ox by bit.bio

opti-ox (optimised inducible overexpression) is our deterministic cell programming technology. It gives us the ability to faithfully execute genetic programs in human stem cells.

Transcription factors controlled by an inducible genetic switch are integrated into genomic safe harbour sites in the stem cell genome. Genomic safe harbour sites protect the integrity of the cell and the inserted program by avoiding gene silencing.

As every pluripotent stem cell in a population contains the same inducible program in their genomic safe harbour sites, we can precisely and consistently reprogram the population into a chosen cell identity at a commercial scale.

Explore our cell identity coding platform