Poster
Advancing drug discovery: leveraging CRISPR-Ready ioMicroglia for functional genomics studies
In this scientific poster, first presented at ISSCR 2024, CRISPR-Ready ioMicroglia are characterised, including cell morphology and gene expression profiles, functional Cas9 expression, single gene knockout efficiency, and applicability to pooled CRISPR screens.
In this scientific poster, first presented at ISSCR 2024, CRISPR-Ready ioMicroglia are characterised, including cell morphology and gene expression profiles, functional Cas9 expression, single gene knockout efficiency, and applicability to pooled CRISPR screens.
Microglia play a pivotal role in maintaining neural health, defending against pathogens, and maintaining the brain’s functional equilibrium. Leveraging bit.bio’s deterministic programming technology, we introduce CRISPR-Ready ioMicroglia for CRISPR/Cas9-based knockout screening.
In this poster, you will explore data showing:
- How CRISPR-Ready ioMicroglia have been engineered to constitutively express Cas9, which is functional from day 1 post thaw, and retain all defining features of ioMicroglia, such as key markers expression and cytokine secretion profile.
- How the cells demonstrate >84% knockout efficiency at protein level using a guide RNA (sgRNA) targeting beta-2 microglobulin (B2M).
- The cells applicability to knockout screens, including data from a screen to uncover genes involved in microglia activation in response to stimuli.
Schmidt et al.,
bit.bio
2024
bit.bio
2024