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Protocol overview
Introduction
This protocol describes the plate preparation and co-culture of wild-type ioGlutamatergic Neurons and rat astrocytes for analysis using the MaxTwo High-Density Microelectrode Array (HD-MEA) System (MaxWell Biosystems AG). Optimisation was performed on MaxTwo 6-Well Plate and 24-well plate formats. The protocol is also applicable to any of the ioGlutamatergic Neurons disease model cells.
When co-cultured with astrocytes, glutamatergic neurons show enhanced maturity and synaptic activity. Astrocytes support stable network activity and minimise neuronal stress and cell death, enabling MEA assays over extended periods.
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