ioGlutamatergic Neurons and related disease models

This user manual is applicable to ioGlutamatergic Neurons  (wild type) and ioGlutamatergic Neurons disease model cells carrying mutations in HTT, TDP-43 (TARDBP), MAPT, PRKN, SNCA, APP, PSEN1, PINK1 and GBA.

The ioGlutamatergic Neurons mature rapidly into functional glutamatergic neurons after revival in the recommended, fully disclosed, medium. They are delivered in a convenient cryopreserved format and provide a homogeneous and reproducible model for human excitatory neurons.

The user manual provides full details for phases 1 and 2 in plate formats from 6- to 384-wells and includes a protocol for co-culture with astrocytes.

Induction

Carried out at bit.bio before distribution.

Phase 1 - Stabilisation (day 0 - 4)

ioGlutamatergic Neurons are revived at the user’s laboratory using the recommended medium.

Phase 2 - Maintenance (day 4 onwards)

Depending on assay requirements, the ioGlutamatergic Neurons can be cultured for different lengths of time in the maintenance medium.

Complete the form to download the ioGlutamatergic Neurons and disease model cells user manual.