Addressing the Reproducibility Crisis | Driving Genome-Wide Consistency in Cellular Reprogramming

Biological variance is a compounding factor in the use of in vitro iPSC-derived cells, where many unknown quantities with iPSC donor or culture methods can affect experimental output and data reproducibility. This cell variability means scientists often struggle to generate the same experimental results over time or fail to reproduce another scientist's experiments using the ‘same’ methods and the ‘same’ cells. 

In this webinar, Dr. Ania Wilczynska will discuss a new method for making human iPSC-derived cells, opti-ox precision cellular reprogramming technology.  This technology synchronously reprograms iPSCs with remarkable genome-wide consistency. Through transcriptome analysis, Ania will demonstrate that opti-ox reprogrammed iPSC-derived cells show minimal biological variance between users, manufacturing lots and time periods.

You’ll discover how this long-term cellular consistency can enable researchers to investigate functions of genes or responses to various compounds in great detail, using a human-relevant model system.

Learning outcomes:

• Understand the factors impacting experimental variance when using iPSC-derived cells.
• Explore a new method of cell generation called opti-ox, which enables precise reprogramming of iPSCs into defined, functional cell types.
• Discover the impact of opti-ox technology on cell consistency.
• Hear how this technology has been used for consistent and precise reprogramming of iPSCs to glutamatergic neurons, GABAergic neurons, microglia and sensory neurons.