cat no | ioEA1027 Early Access
ioMotor Neurons have been precision reprogrammed from human induced pluripotent stem cells (iPSC) using opti-ox™ technology. Within days, cells convert consistently to defined, functional motor neurons, showing the expression of key lower motor neuron marker genes MNX1(HB9), FOXP1, ISL2 and cholinergic markers CHAT & SLC18A3 (VAChT) by day 4.
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ioMotor Neurons have been extensively characterised, indicating a spinal motor neuron identity (cervical region) confirmed through FOXP1, ISL2, MNX1 and HOX gene expression that is measurable just 4 days post-revival. From day 14, >80% cells express MNX1 and show functional neuronal activity in astrocyte co-culture that is in line with primary motor neurons cultured in similar conditions. Functional activity was also evaluated in monoculture, demonstrating no significant activity, as expected; indicating that the cells form a highly pure and homogenous neuronal population unable to undertake synaptogenesis without glial cells.
ioMotor Neurons represent an accurate in vitro model of lower motor neurons (indicated spinal – cervical region identity), enabling scientists to build physiological relevance into their experiments at scales from single cell analysis to high content imaging, helping bridge translational gaps in motor neuron disease research and neurotoxicology.
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per vial
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Functional
Functional neuronal networks are detected in co-culture with astrocytes from day 14.
Quick and easy
Within 4 days post revival cells are ready for experimentation, displaying motor neuronal morphology without clumping.
Defined
>80% cells express key lower motor neuron markers indicating a spinal motor neuron identity (cervical region). >99.9% neuronal population.
ioMotor Neurons acquire a rapid motor neuronal phenotype, without clumping
ioMotor Neurons form a homogenous neuronal network by day 4
Immunocytochemistry shows protein expression of key motor neuron markers
Bulk RNA-sequencing exhibits a HOX gene signature indicative of a spinal motor neuron (cervical region) identity
Single cell RNA-sequencing shows ioMotor Neurons express key spinal motor neuron markers, >80% of cells express MNX1 on day 14
Bulk RNA-sequencing demonstrates high batch-to-batch consistency of ioMotor Neurons
Do more with every vial
Cell arrive ready to plate
Vaquero, et al
bit.bio
2023
Prof Roger Pedersen | Adjunct Professor and Senior Research Scientist at Stanford University
Dr Thomas Moreau | Director of Cell Biology Research | bit.bio