ioGlutamatergic Neurons and ioAstrocytes are human induced pluripotent stem cell (iPSC)-derived glutamatergic neurons and astrocytes, deterministically programmed using opti-ox™ technology. This protocol describes a method for co-culturing ioGlutamatergic Neurons with ioAstrocytes to facilitate research into complex neuroglial interactions and improve neuronal network activity in multi-electrode array (MEA) systems. 

The full protocol includes simple step-by-step instructions for cell culture, recipes for media and information on required equipment and reagents.

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