cat no | io1009
ioGlutamatergic Neurons
MAPT N279K/WT
Human iPSC-derived FTD disease model
A rapidly maturing, consistent and scalable isogenic system to study frontotemporal dementia (FTD).
ioGlutamatergic Neurons MAPT N279K/WT are opti-ox precision reprogrammed glutamatergic neurons carrying a genetically engineered heterozygous N279K mutation in the MAPT gene encoding the tau protein.
Place your order
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
Benchtop benefits
Disease related phenotype
High content ICC image analysis shows hyperphosphorylation of tau compared to the wild-type control.
Make True Comparisons
Pair the ioDisease Model Cells with the genetically matched wild-type ioGlutamatergic Neurons to directly investigate the impact of mutant tau protein on disease.
Quick
The disease model cells and isogenic control are experiment ready as early as 2 days post revival, and form structural neuronal networks at 11 days.
Disease-related phenotype
Hyperphosphorylation of tau observed in the disease model cells compared to the isogenic control
ioGlutamatergic Neurons disease model cells carrying MAPT P301S/P301S, MAPT N279K/WT and MAPT N279K/N279K mutations show hyperphosphorylation when compared to the isogenic control ioGlutamatergic Neurons (WT) at day 21. The bar graphs show total Tau, pTau217/total Tau, pTau202/5/total Tau or pTau404/total Tau in cell bodies, analysed by immunocytochemistry. Statistical analyses performed on 5 cellular replicates in the same plate. Bars showing mean, error bars showing Standard Deviation. Statistics calculated by one way ANOVA and Tukey posthoc analysis. Data courtesy of Charles River Laboratories.
Highly characterised and defined
ioGlutamatergic Neurons MAPT N279K/WT express neuron-specific markers comparably to the isogenic control
Immunofluorescent staining on post-revival day 11 demonstrates similar homogenous expression of pan-neuronal proteins MAP2 and TUBB3 (upper panel) and glutamatergic neuron-specific transporter VGLUT2 (lower panel) in ioGlutamatergic Neurons MAPT N279K/WT compared to the isogenic control. 100X magnification.
ioGlutamatergic Neurons MAPT N279K/WT form structural neuronal networks by day 11
ioGlutamatergic Neurons MAPT N279K/WT mature rapidly and form structural neuronal networks over 11 days, when compared to the isogenic control. Day 1 to 11 post thaw; 100X magnification.
ioGlutamatergic Neurons MAPT N279K/WT demonstrate gene expression of neuronal-specific and glutamatergic-specific markers following deterministic programming
Gene expression analysis demonstrates that ioGlutamatergic Neurons MAPT N279K/WT and the isogenic control (WT) lack the expression of pluripotency markers (NANOG and OCT4) at day 11, whilst robustly expressing pan-neuronal (TUBB3 and SYP) and glutamatergic specific (VGLUT1 and VGLUT2) markers, as well as the glutamate receptor GRIA4. Gene expression levels were assessed by RT-qPCR. Data is shown relative to the parental hiPSC control (iPSC Control), normalised to HMBS. Data represents day 11 post-revival samples; n=2 biological replicates
Disease-related MAPT is expressed in ioGlutamatergic Neurons MAPT N279K/WT following deterministic programming
Gene expression analysis demonstrates that ioGlutamatergic Neurons MAPT N279K/WT and the isogenic control (WT) express the MAPT gene encoding the tau protein. Gene expression levels were assessed by RT-qPCR (data expressed relative to the parental hiPSC control (iPSC Control), normalised to HMBS). Data represents day 11 post-revival samples.
Cells arrive ready to plate
ioGlutamatergic Neurons MAPT N279K/WT are delivered in a cryopreserved format and are programmed to mature rapidly upon revival in the recommended media. The protocol for the generation of these cells is a two-phase process: Phase 1, Stabilisation for 4 days; Phase 2, Maintenance, during which the neurons mature. Phases 1 and 2 after revival of cells are carried out by the customer.
Industry leading seeding density
Do more with every vial
The recommended minimum seeding density is 30,000 cells/cm2, compared to up to 250,000 cells/cm2 for other similar products on the market. One small vial can plate a minimum of 0.7 x 24-well plate, 1 x 96-well plate, or 1.5 x 384-well plates. One large vial can plate a minimum of 3.6 x 24-well plates, 5.4 x 96-well plates, or 7.75 x 384-well plates. This means every vial goes further, enabling more experimental conditions and more repeats, resulting in more confidence in the data.
(LinkedValues: [{hs_name=Generating publishable neuroscience research in 12 weeks with ioGlutamatergic Neurons, hs_id=161746296926, hs_path=generating-publishable-neuroscience-research-in-12-weeks-with-glutamatergic-neurons, button_label=null, button_link=null, type={label=Case study, value=Case study}, thumbnail={alt_text=, width=2400, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Compressed%20Photos%20and%20Gifs/bit.bio_case_study_Pic0012_WebRes.jpg, height=1600}, year={label=2024, value=2024}, summary=<p>Professor Deepak Srivastava</p>
<p>Professor of Molecular Neuroscience and Group Leader, MRC Centre for Developmental Disorders</p>
<p>King’s College London </p>, date_published=1710720000000, sort_date=1705276800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>V2</p>
<p>2024</p>
<p>bit.bio</p>, listing_button_label=Download}, {hs_name=ioGlutamatergic Neurons , hs_id=161746296923, hs_path=glutamatergic-neurons, button_label=View brochure, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Brochures/bit.bio-ioGlutamatergic%20Neurons-Brochure.pdf, type={label=Brochure, value=Brochure}, thumbnail={alt_text=, width=2550, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Discover%20ioCells/iowild-type/bit.bio-ioGlutamatergic-Neurons-Day-14-Beta-III-Tubulin-staining-banner.jpg, height=1791}, year={label=2024, value=2024}, summary=<p>bit.bio</p>, date_published=1713312000000, sort_date=1713312000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioAstrocytes, value=ioAstrocytes}, {label=Product information, value=Product information}], media_contact=null, listing_button_label=Download}, {hs_name=High Content Analysis of 2D and 3D cellular models for target and phenotypic drug discovery, hs_id=161961134487, hs_path=charles-river-poster-high-content-analysis-of-cellular-models, button_label=View poster, button_link=https://bit.bio/hubfs/BitBio_April2021/pdf%20link/2020_Lachize-et-al_High-Content-Analysis-of-2D-and-3D-Cellular-Models_Charles-River.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2020, value=2020}, summary=<p>Lachize, et al</p>
<p>Courtesy of Charles River Laboratories</p>
<p>2020</p>, date_published=1706313600000, sort_date=1602806400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=null, listing_button_label=View}, {hs_name=Development and characterisation of a robust in vitro disease model to study tauopathies, hs_id=161961134488, hs_path=ftd-mapt-phenotype-poster-charles-river-mar23, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/SFN2022-Development-and-characterization-of-a-robust-in-vitro-disease-model-to-study-tauopathies.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1000, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/MAPT%20P301S/MAPT%20P301SP301S%20final%20homepage%20image.jpg, height=1400}, year={label=2022, value=2022}, summary=<p>Ritsma, et al</p>
<p>Charles River Laboratories & bit.bio</p>
<p>2022</p>, date_published=1708646400000, sort_date=1678924800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}], media_contact=null, listing_button_label=View}, {hs_name=Rapid and consistent generation of functional microglia from reprogrammed hiPSCs to study neurodegeneration and neuroinflammation, hs_id=161961134491, hs_path=poster-rapid_and_consistent_generation_of_functional_ipsc_derived_microglia, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/bitbio-ioMicroglia_poster-V7-press_SfN%202022.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1504, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Emails/Header%20images/Header%20Images%20-%20ICC%20only/Microglia%20blog%20email%20header%20format.png, height=850}, year={label=2022, value=2022}, summary=<p>Raman, et al</p>
<p>bit.bio</p>
<p>2022</p>, date_published=1709251200000, sort_date=1669680000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioMicroglia, value=ioMicroglia}], media_contact=null, listing_button_label=View}, {hs_name=Modelling neurodegeneration: Human isogenic system to study FTD & ALS, hs_id=161961134492, hs_path=ftd-als-mapt-tdp-43-disease-models-adpd-2023-poster-mar23, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/bitbio_ioGlutamatergic%20Neurons%20MAPT%20TDP-43%20Disease%20Model%20poster_v2.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1966, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/MAPT%20N279K/N279K%20MAP2%20brightfield%20compressed%20rotated.png, height=1966}, year={label=2023, value=2023}, summary=<p>Oosterveen, et al</p>
<p>bit.bio & Charles River Laboratories</p>
<p>2023</p>, date_published=1709424000000, sort_date=1687478400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}], media_contact=null, listing_button_label=View}, {hs_name=Generation and characterisation of a panel of human iPSC-derived neurons and microglia carrying early and late onset relevant mutations for Alzheimer’s disease, hs_id=161961134495, hs_path=slas2024-ad-poster, button_label=null, button_link=null, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1800, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/Microglia%20hero%202%20resize%20brighten%20v2%20compress.png, height=1800}, year={label=2024, value=2024}, summary=Smith, et al. <br>bit.bio<br>2024, date_published=1711065600000, sort_date=1708300800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}], media_contact=Smith et al. <br>bit.bio<br>2024, listing_button_label=Download}, {hs_name=CRISPR and the Art of Perturbation Screening: Unbiased functional genomic screening meets the best human cellular models, hs_id=161968263475, hs_path=crispr-and-the-art-of-perturbation-screening-unbiased-functional-genomic-screening-meets-the-best-human-cellular-models, button_label=Explore ioCRISPR-Ready Cells, button_link=https://www.bit.bio/iocrispr-ready, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/CRISPR%20and%20the%20Art%20of%20Perturbation%20Screening-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Kam Dhaliwal <span>| </span>SVP Strategic Alliances | bit.bio</p>
<p><br>Talk at ELRIG CRISPR in Drug Discovery</p>, date_published=1706140800000, sort_date=1636070400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioSensory Neurons, value=ioSensory Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Consistent and scalable human iPSC-derived cells for in vitro disease modelling and drug discovery, hs_id=161968263476, hs_path=consistent-and-scalable-human-ipsc-derived-cells-for-in-vitro-disease-modelling-and-drug-discovery, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=900, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/DDD_banner_square.jpeg, height=652}, year={label=2020, value=2020}, summary=<p>Kam Dhaliwal <span>| </span> SVP Strategic Alliances | bit.bio<br>Dr Thomas Moreau <span>|</span> Head of Research | bit.bio</p>
<p><br>Talk at ELRIG Drug Discovery Digital</p>, date_published=1706400000000, sort_date=1600905600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}], media_contact=null, listing_button_label=Watch now}, {hs_name=Precision Cellular Reprogramming for Scalable and Consistent Human Neurodegenerative Disease Models, hs_id=161968263479, hs_path=precision-cellular-reprogramming-for-scalable-and-consistent-human-neurodegenerative-disease-models, button_label=Explore ioDisease Model Cells, button_link=https://www.bit.bio/iodisease-models, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Tech%20Nets%20online%20symposium%20header%20(1).png, height=1260}, year={label=2023, value=2023}, summary=<p>Madeleine Garrett | Field Application Specialist | bit.bio</p>, date_published=1709078400000, sort_date=1681344000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioMicroglia, value=ioMicroglia}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}, {label=ioMotor Neurons, value=ioMotor Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Industrialising Cellular Reprogramming: Leveraging opti-ox Technology to Manufacture Human Cells with Unprecedented Consistency, hs_id=161968263480, hs_path=industrialising-cellular-reprogramming-leveraging-opti-ox-technology-to-manufacture-human-cells-with-unprecedented-consistency, button_label=null, button_link=null, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1024, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Resources/video/Squarespace+Thumbnail+Template+-+AM23-01-01-01.png, height=768}, year={label=2023, value=2023}, summary=<p>Innovation showcase talk at ISSCR</p>
<p>Marius Wernig MD, PhD | Stanford </p>
<p>Mark Kotter, MD, PhD | bit.bio</p>, date_published=1709683200000, sort_date=1688083200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=CRISPR-Ready ioCells, value=CRISPR-Ready ioCells}], media_contact=<p>V1<br><br>2023<br><br>ISSCR 2023</p>, listing_button_label=Watch now}, {hs_name=ioGlutamatergic Neurons Wild Type and related disease models | User Manual, hs_id=161968263487, hs_path=glutamatergic-neurons, button_label=null, button_link=null, type={label=User manual, value=User manual}, thumbnail={alt_text=bit-bio ioGlutamatergic Neurons Day 14 Beta III Tubulin staining copy, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/ioGlutamatergic%20Neurons%20in%20Drug%20Discovery/bit-bio%20ioGlutamatergic%20Neurons%20Day%2014%20Beta%20III%20Tubulin%20staining%20copy.jpeg, height=1200}, year={label=2024, value=2024}, summary=<p>V11</p>
<p>bit.bio</p>
<p>2024</p>, date_published=1707955200000, sort_date=1713225600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}, {label=ioAstrocytes, value=ioAstrocytes}, {label=Product information, value=Product information}], media_contact=<p><span>V11</span></p>
<p><span>bit.bio</span></p>
<p><span>2024</span></p>, listing_button_label=Download}, {hs_name=Partnering with Charles River to advance CNS drug discovery with ioGlutamatergic Neurons, hs_id=161968263497, hs_path=in-conversation-with-charles-river-video-part-2, button_label=null, button_link=null, type={label=Video, value=Video}, thumbnail={alt_text=, width=687, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/CRL%20video%20posts/crl%202%20webpage%20image-2.png, height=476}, year={label=2021, value=2021}, summary=<p>Dr Marijn Vlaming | Head of Biology, et al.</p>
<p>Charles River & bit.bio</p>, date_published=1705017600000, sort_date=1637107200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch }, {hs_name=In Conversation with Charles River, hs_id=161968263498, hs_path=in-conversation-with-charles-river-video, button_label=Discover our ioCells portfolio, button_link=/discover-iocells, type={label=Video, value=Video}, thumbnail={alt_text=, width=834, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/CRL%20video%20posts/CRL%20video%20%231%20card%20for%20webpage%20(thinner%20gradient%20line)-1.png, height=590}, year={label=2021, value=2021}, summary=<p>Dr Marijn Vlaming <span>|</span> Head of Biology<br>Charles River</p>, date_published=1705363200000, sort_date=1636675200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=null, listing_button_label=Watch}, {hs_name=Culturing Glutamatergic Neurons | How-to Video, hs_id=161966111374, hs_path=how-to-culture-ioglutamatergic-neurons, button_label=Download the user manual, button_link=/resources/ioglutamatergic-neurons-user-manual, type={label=Video tutorial, value=Video tutorial}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/How%20to%20prepare%20tissue%20culture%20vessels%20with%20PDL-Geltrex-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Dr Kaiser Karim <span>|</span> Scientist<br>bit.bio</p>, date_published=1705104000000, sort_date=1637020800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch now}, {hs_name=Preparing Culture Vessels for Glutamatergic Neurons | How-to Video, hs_id=161966111375, hs_path=how-to-prepare-tissue-culture-vessels-with-pdl-geltrex, button_label=Watch the next video, button_link=/resources/how-to-culture-ioglutamatergic-neurons, type={label=Video tutorial, value=Video tutorial}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/How%20to%20culture%20ioGlutamatergic%20Neurons-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Dr Kaiser Karim <span>|</span> Scientist<br>bit.bio</p>, date_published=1705190400000, sort_date=1636934400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch now}, {hs_name=Modelling neurodevelopment | Investigating the impact of maternal immune activation on neurodevelopment using human iPSC-derived cells, hs_id=161968263519, hs_path=modelling-neurodevelopment-webinar-2022, button_label=Explore ioGlutamatergic Neurons, button_link=https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/product-page/Colour%20webinar%20with%2060x%20Map2-com.jpg, height=732}, year={label=2022, value=2022}, summary=<p><span>Dr Deepak Srivastava | King’s College London<br><br></span><span></span></p>, date_published=1707436800000, sort_date=1649376000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Rethinking Developmental Biology With Cellular Reprogramming, hs_id=161968263524, hs_path=rethinking-developmental-biology-with-cellular-reprogramming, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Upcoming%20Webinars/Tech%20Nets%202023/bit.bio_ioGlutamatergic%20Neurons_20xMAP2(red)Hoescht(blue)_day12v2.png, height=1260}, year={label=2023, value=2023}, summary=<p>Mark Kotter | CEO and founder | bit.bio</p>
<p>Marius Wernig | Professor Departments of Pathology and Chemical and Systems Biology | Stanford University</p>, date_published=1709164800000, sort_date=1681776000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}, {label=Cell therapy, value=Cell therapy}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}], media_contact=null, listing_button_label=Watch now}, {hs_name=Addressing the Reproducibility Crisis | Driving Genome-Wide Consistency in Cellular Reprogramming, hs_id=161968263525, hs_path=addressing-the-reproducibility-crisis-driving-genome-wide-consistency-in-cellular-reprogramming, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Upcoming%20Webinars/Tech%20Nets%2019th%20July%202023/bit.bio_ioGlutamatergic%20Neurons_60xMAP2(red)Hoescht(blue)TUBB3(blue)_day4.jpg, height=1260}, year={label=2023, value=2023}, summary=<p>Dr Ania Wilczynska | Head of Computational Genomics | Non-Clinical | bit.bio</p>, date_published=1709769600000, sort_date=1689724800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=CRISPR-Ready ioGlutamatergic Neurons, value=CRISPR-Ready ioGlutamatergic Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Running Large-Scale CRISPR Screens in Human Neurons, hs_id=161968263528, hs_path=running-large-scale-crispr-screens-in-human-neurons, button_label=null, button_link=null, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Emails/Header%20images/Header%20Images%20-%20ICC%20only/Running%20Large-Scale%20CRISPR%20Screens%20in%20Human%20Neurons_email%20header.png, height=678}, year={label=2023, value=2023}, summary=<p>Emmanouil Metzakopian | Vice President, Research and Development | bit.bio</p>
<p>Javier Conde-Vancells | Director Product Management | bit.bio</p>, date_published=1710460800000, sort_date=1700524800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=CRISPR-Ready ioCells, value=CRISPR-Ready ioCells}, {label=CRISPR-Ready ioGlutamatergic Neurons, value=CRISPR-Ready ioGlutamatergic Neurons}, {label=CRISPR-Ready ioMicroglia, value=CRISPR-Ready ioMicroglia}], media_contact=null, listing_button_label=Watch now}, {hs_name=Human iPSC-Based Models of Glial Cells for Studying Neurodegenerative Disease, hs_id=183096620633, hs_path=human-ipsc-based-models-of-glial-cells-for-studying-neurodegenerative-disease, button_label=null, button_link=null, type={label=Webinar, value=Webinar}, thumbnail={alt_text=bitbio-oligodendrocyte-like-cells-O4-MBP-immunocytochemistry, width=1000, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioOligodendrocyte-like%20cells/bitbio-oligodendrocyte-like-cells-O4-MBP-immunocytochemistry.jpg, height=1350}, year={label=2024, value=2024}, summary=Valentina Fossati, PhD | Senior Research Investigator | The New York Stem Cell Foundation<br><br>Inês Ferreira | Senior Product Manager | bit.bio, date_published=1732233600000, sort_date=1732233600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}], media_contact=null, listing_button_label=Watch now}])
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Human iPSC-Based Models of Glial Cells for Studying Neurodegenerative Disease
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ioGlutamatergic Neurons
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ioGlutamatergic Neurons Wild Type and related disease models | User Manual
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Generation and characterisation of a panel of human iPSC-derived neurons and microglia carrying early and late onset relevant mutations for Alzheimer’s disease
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Generating publishable neuroscience research in 12 weeks with ioGlutamatergic Neurons
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Running Large-Scale CRISPR Screens in Human Neurons
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Addressing the Reproducibility Crisis | Driving Genome-Wide Consistency in Cellular Reprogramming
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Cell culture hacks | human iPSC-derived glutamatergic neurons
Read this blog on glutamatergic neuron cell culture for our top tips on careful handling, cell plating and media changes to achieve success from the outset.
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT N279K/N279K
cat no | io1014
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT P301S/WT
cat no | io1015
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT P301S/P301S
cat no | io1008
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ioWild Type Cells
ioGlutamatergic Neurons
cat no | io1001
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CRISPR-Ready ioCells
CRISPR-Ready ioGlutamatergic Neurons
cat no | io1090
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ioDisease Model Cells
ioGlutamatergic Neurons HTT 50CAG/WT
cat no | ioEA1004
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ioDisease Model Cells
ioGlutamatergic Neurons TDP-43 M337V/WT
cat no | ioEA1006
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ioDisease Model Cells
ioGlutamatergic Neurons PRKN R275W/WT
cat no | io1013
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ioDisease Model Cells
ioGlutamatergic Neurons TDP-43 M337V/M337V
cat no | ioEA1005
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ioDisease Model Cells
ioGlutamatergic Neurons PRKN R275W/R275W
cat no | io1020
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Wild Type and Isogenic Disease Model cells: A true comparison.
Further your disease research by pairing our wild type cells with isogenic disease models.
