Human-Induced Glutamatergic Neurons
e001 | Formerly known as ioNEURONS/glut
ioGlutamatergic Neurons have been reprogrammed from human induced pluripotent stem cells (iPSC) using our precise reprogramming technology: opti-ox¹ (optimized inducible overexpression). Human stem cells, within days, convert into consistent, mature, functional glutamatergic neurons providing a high quality human model for the study of neurological activity and disease.
ioGlutamatergic Neurons consist mainly of glutamatergic neurons (>80%) characterised by the expression of the glutamate transporter genes VGLUT1 and VGLUT2. The minor remaining fraction of the neuronal population express marker genes of cholinergic neurons. A bulk RNA-seq analysis shows that ioGlutamatergic Neurons have a rostral CNS identity and express the classical cortical marker genes FOXG1 and TBR1 (data not shown).
Ready-to-culture cells are suitable as models for research in cell-type specific biology, target validation and drug screening in pharmaceutical R&D, and toxicology testing.
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Batch to batch reproducibility and homogeneity create a stable human model for excitatory neuronal activity and disease.
Ready for experimentation as early as 2 days post revival and form functional neuronal networks at 17 days.
Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.
Cells arrive programmed to rapidly mature upon revival. One medium required in a two-step protocol.
ioGlutamatergic Neurons generated by NGN2-driven reprogramming of iPSCs using opti-ox technology
Video capturing the rapid morphological changes from iPSCs upon induction of NGN2 expression using opti-ox cellular reprogramming. 7 day time course.
ioGlutamatergic Neurons express glutamatergic neuron-specific markers
ioGlutamatergic Neurons after revival over the course of the first 11 days
ioGlutamatergic Neurons display neuronal activity that matures over-time
ioGlutamatergic Neurons show good suitability for high-throughput screening in 384-well format plates
Cells arrive ready to plate
Cost effective and flexible
Catalogue number: e001 Manufacture site: United Kingdom Sizes: Small & Large Product use: These cells are for research use only Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments Shipping info: Dry Ice Note: Limited Use License | Statement of Use