cat no | io1001
ioGlutamatergic Neurons
Human iPSC-derived glutamatergic neurons
ioGlutamatergic Neurons are deterministically programmed from human induced pluripotent stem cells (iPSC) using opti-ox technology. Within days, cells convert consistently to mature, functional glutamatergic neurons characterised by >80% expression of glutamate transporter genes VGLUT1 and VGLUT2.
Place your order
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
Benchtop benefits
Quick
Ready for experimentation as early as 2 days post revival and form functional neuronal networks at 17 days.
Scalable
Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.
Easy to use
Cells arrive programmed to mature rapidly upon revival. One medium is required in a two-step protocol.
Ready within days
ioGlutamatergic Neurons generated by transcription factor-driven deterministic programming of iPSCs using opti-ox technology
Time-lapse video capturing the rapid and homogeneous neuronal phenotype acquisition upon thawing of cryopreserved ioGlutamatergic Neurons. 7 day time course.
Highly characterised and defined
ioGlutamatergic Neurons express glutamatergic neuron-specific markers
MAP2
VGLUT2
DAPI
%20MAP2(r)%20DAPI(b).jpeg?width=213&height=213&name=bit-bio%20ioGlutamatergic%20Neurons%20-%20Day%2011%20-%20MERGE%20VGLUT2(g)%20MAP2(r)%20DAPI(b).jpeg)
MERGE
Immunofluorescent staining on day 11 post-revival demonstrates homogenous expression of the pan-neuronal protein, MAP2 and glutamatergic neuron-specific transporter, VGLUT2.
ioGlutamatergic Neurons form structural neuronal networks by day 11
Day 1
Day 4
Day 7
Day 11
ioGlutamatergic Neurons mature rapidly and form structural neuronal networks over 11 days. Day 1 to 11 post thawing; 100X magnification.
Whole transcriptome analysis demonstrates high lot-to-lot consistency across three manufactured lots of ioGlutamatergic Neurons
Bulk RNA-sequencing analysis was performed on three different lots of ioGlutamatergic Neurons on day 0, day 11 and day 18 post-revival. (A) A principal component analysis (PCA) to assess gene expression variance between three different manufactured lots showed a tight clustering of the samples at each timepoint, demonstrating high consistency between these lots. This lot-to-lot consistency of ioGlutamatergic Neurons will help reduce experimental variation and increase the reproducibility of experiments. (B) PCA without the parental non-induced hiPSC samples, highlighting the tight clustering of the day 11 as well as day 18 samples of the three different lots. (C) Differential expression test reveals no statistically significant differentially expressed (DE) genes across the three lots at day 11 (|logFC| > 0.5 and FDR < 0.01).
Colours represent the three lots of products; shapes represent the parental non-induced hiPSC line and different timepoints.
Expression levels for specific genes of interest can be requested by contacting our team at technical@bit.bio.
High lot-to-lot consistency is demonstrated by a consistent transcriptomic fingerprint across manufactured lots of ioGlutamatergic Neurons
Single cell RNA-sequencing analysis was performed on three different lots of ioGlutamatergic Neurons on day 11. UMAP plots represent the cell-to-cell variation in gene expression profiles of cells, each dot representing an individual cell. Cells from each of the three lots are equally distributed across the body of the plot. Merging the UMAP plots creates a tight overlay, showing a strong transcriptional relationship between cells from three independently manufactured lots of ioGlutamatergic Neurons. Gene expression was assessed by 10x Genomics scRNA-sequencing.
Single cell ATAC-sequencing shows a consistent transcriptomic fingerprint demonstrating high lot-to-lot consistency across manufactured lots of ioGlutamatergic Neurons
Single cell ATAC-sequencing analysis was performed on three different lots of ioGlutamatergic Neurons on day 11. Single cell ATAC-sequencing reveals regions of open chromatin to understand the gene regulatory landscape of individual cells. UMAP plots represent the cell-to-cell variation in chromatin accessibility of the cells, each dot representing a single cell. Cells from each of the three lots are equally distributed across the body of the plot. Merging the UMAP plots creates a tight overlay, showing a strong transcriptional relationship between cells from three independently manufactured lots of ioGlutamatergic Neurons. Gene expression was assessed by 10x Genomics scRNA-sequencing.
Rapid gain of functional activity
ioGlutamatergic Neurons display neuronal activity that matures over time
The function of ioGlutamatergic Neurons was investigated using the MaxTwo HD-MEA System.
The Axon Tracking Assay (left) shows examples of reconstructed axonal paths of travelling action potentials of individual iPSC-derived glutamatergic neurons. The assay reveals the spatial propagation of the neuronal action potential from the soma to distant axonal branches.
Total axon length (middle) and firing rate (right) increase over time, indicating that the cells are maturing. ioGlutamatergic Neurons were cultured with human iPSC-derived astrocytes.
Data courtesy of Charles River Laboratories and MaxWell Biosystems.
Rapid maturation of ioGlutamatergic Neurons leads to synchronised network activity by day 31
Raster plots generated using the MaxTwo HD-MEA System show the development of the neuronal network over time.
The plots show the dynamics of the network activity using 1,024 active electrodes. Each row represents an individual electrode and each blue dot indicates a spike detected at that electrode over a period of 300 seconds.
Spontaneous activity is observed at DIV 7. Clear synchronised bursting activity is observed by DIV 31, represented by blue vertical lines, followed by an overall drop in activity, seen as white lines.
Download our poster to see additional data that shows how ioGABAergic Neurons form functional neuronal networks with ioGlutamatergic Neurons in the presence of astrocytes, and how the tri-culture responds to bicuculline and diazepam.
ioGlutamatergic Neurons offer a rapidly maturing functional system that can be used to assess neuronal networks and the impact of a drug treatment or intervention.
Data courtesy of Charles River Laboratories and MaxWell Biosystems.
Robust and scalable cells for screening applications
ioGlutamatergic Neurons show good suitability for high-throughput screening in 384-well format plates
Cytotoxicity CellTiter-Glo®️ (CTG) and TR-FRET (HTRF®️) assays for AKT serine/threonine kinase 1 (AKT) and Huntingtin (HTT) proteins were performed on ioGlutamatergic Neurons in 384-well plates treated with tool compound (cmp) at day 9 post-revival. Compound titration results in a concentration response curve for all three assays (mean±sd of 2 replicates). CTG assay on ioGlutamatergic Neurons shows an excellent average signal-to-background ratio and high suitability for HTS. HTRF assays on ioGlutamatergic Neurons show lower signals but with low variability, and could therefore also provide a suitable platform for HTS.
Data courtesy of Charles River Laboratories.
ioGlutamatergic Neurons offer a robust, physiologically-relevant model for efficacy screening of candidate ASOs
Positive and negative control antisense oligonucleotides (ASOs) with gapmer chemistry were introduced into glutamatergic neurons by gymnosis. RT-qPCR was used to measure ASO-induced gene knockdown.
- Strong separation of the assay signal for positive control (blue) and negative control (orange) ASOs was observed for all plates tested (A).
- The positive control ASO induced ~90% knockdown of the target gene, shown by a decrease in the target gene expression (A) and higher Cp (or Ct) values for the target gene, indicating lower initial amount of the target sequence (B).
- There was no effect of the control ASOs on housekeeping gene expression as compared to vehicle-transfected controls (C).
- No marked intra- or inter-plate variability was observed between positive and negative control ASOs (A-C).
Data courtesy of Charles River Laboratories.
Industry leading seeding density
Do more with every vial
The recommended minimum seeding density is 30,000 cells/cm2, compared to up to 250,000 cells/cm2 for other similar products on the market. One small vial can plate a minimum of 0.7 x 24-well plate, 1 x 96-well plate, or 1.5 x 384-well plates. One large vial can plate a minimum of 3.6 x 24-well plates, 5.4 x 96-well plates, or 7.75 x 384-well plates. This means every vial goes further, enabling more experimental conditions and more repeats, resulting in more confidence in the data.
Easy culturing
Cells arrive ready to plate
ioGlutamatergic Neurons are delivered in a cryopreserved format and are programmed to mature rapidly upon revival in the recommended media. The protocol for the generation of these cells is a two-phase process: 1. Stabilisation for 4 days 2. Maintenance during which the neurons mature.
(LinkedValues: [{hs_name=Developing next-generation in vitro phenotypic assays for Huntington’s disease by combining a precision reprogrammed hiPSC-derived disease model with high-density microelectrode arrays, hs_id=161727133271, hs_path=ioglutamatergic-neurons-htt-maxwell-crl, button_label=null, button_link=null, type={label=Application note, value=Application note}, thumbnail={alt_text=, width=600, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons%20HTT%2050CAGWT/a-HTT50CAGWT_Overlay__TUBB3(G)_MAP2(R)_DAPI(B)_%20(1).png, height=378}, year={label=2022, value=2022}, summary=<p>bit.bio | MaxWell Biosystems | Charles River Laboratories</p>
<p>2022</p>, date_published=1708560000000, sort_date=1668556800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}], media_contact=<p>bit.bio | MaxWell Biosystems | Charles River Laboratories</p>
<p>2022</p>
<p> </p>, listing_button_label=Download}, {hs_name=Sartorius application note - Advanced in vitro Modeling of Human iPSC-derived Neuronal Mono- and Co-cultures with Microglia, hs_id=177620758074, hs_path=sartorius-application-note-advanced-in-vitro-modeling-of-human-ipsc-derived-neuronal-mono-and-co-cultures-with-microglia, button_label=null, button_link=null, type={label=Application note, value=Application note}, thumbnail={alt_text=co culture low density progenitors compressed, width=1732, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/co%20culture%20low%20density%20progenitors%20compressed.png, height=970}, year={label=2024, value=2024}, summary=Trigg et al.,<br>Sartorius<br>2024, date_published=1725580800000, sort_date=1725580800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioMicroglia, value=ioMicroglia}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}], media_contact=Trigg et al.,<br>Sartorius<br>2024, listing_button_label=Download}, {hs_name=Producing 3D Neuronal Microtissues for Preclinical Drug Screening using ioGlutamatergic Neurons, hs_id=180505546901, hs_path=glutamatergic-neuron-3d-neuronal-microtissues-inventia, button_label=null, button_link=null, type={label=Application note, value=Application note}, thumbnail={alt_text=bitbio-ioGlutamatergic-Neurons, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2024, value=2024}, summary=V1<br>2024<br>bit.bio<br>Inventia, date_published=1728345600000, sort_date=1728345600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioAstrocytes, value=ioastrocytes}], media_contact=V1<br>2024<br>bit.bio<br>Inventia, listing_button_label=Download}, {hs_name=Generating publishable neuroscience research in 12 weeks with ioGlutamatergic Neurons, hs_id=161746296926, hs_path=generating-publishable-neuroscience-research-in-12-weeks-with-glutamatergic-neurons, button_label=null, button_link=null, type={label=Case study, value=Case study}, thumbnail={alt_text=, width=2400, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Compressed%20Photos%20and%20Gifs/bit.bio_case_study_Pic0012_WebRes.jpg, height=1600}, year={label=2024, value=2024}, summary=<p>Professor Deepak Srivastava</p>
<p>Professor of Molecular Neuroscience and Group Leader, MRC Centre for Developmental Disorders</p>
<p>King’s College London </p>, date_published=1710720000000, sort_date=1705276800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>V2</p>
<p>2024</p>
<p>bit.bio</p>, listing_button_label=Download}, {hs_name=ioGlutamatergic Neurons , hs_id=161746296923, hs_path=glutamatergic-neurons, button_label=View brochure, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Brochures/bit.bio-ioGlutamatergic%20Neurons-Brochure.pdf, type={label=Brochure, value=Brochure}, thumbnail={alt_text=, width=2550, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Discover%20ioCells/iowild-type/bit.bio-ioGlutamatergic-Neurons-Day-14-Beta-III-Tubulin-staining-banner.jpg, height=1791}, year={label=2024, value=2024}, summary=<p>bit.bio</p>, date_published=1713312000000, sort_date=1713312000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioAstrocytes, value=ioAstrocytes}, {label=Product information, value=Product information}], media_contact=null, listing_button_label=Download}, {hs_name=High Content Analysis of 2D and 3D cellular models for target and phenotypic drug discovery, hs_id=161961134487, hs_path=charles-river-poster-high-content-analysis-of-cellular-models, button_label=View poster, button_link=https://bit.bio/hubfs/BitBio_April2021/pdf%20link/2020_Lachize-et-al_High-Content-Analysis-of-2D-and-3D-Cellular-Models_Charles-River.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2020, value=2020}, summary=<p>Lachize, et al</p>
<p>Courtesy of Charles River Laboratories</p>
<p>2020</p>, date_published=1706313600000, sort_date=1602806400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=null, listing_button_label=View}, {hs_name=Development and characterisation of a robust in vitro disease model to study tauopathies, hs_id=161961134488, hs_path=ftd-mapt-phenotype-poster-charles-river-mar23, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/SFN2022-Development-and-characterization-of-a-robust-in-vitro-disease-model-to-study-tauopathies.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1000, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/MAPT%20P301S/MAPT%20P301SP301S%20final%20homepage%20image.jpg, height=1400}, year={label=2022, value=2022}, summary=<p>Ritsma, et al</p>
<p>Charles River Laboratories & bit.bio</p>
<p>2022</p>, date_published=1708646400000, sort_date=1678924800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}], media_contact=null, listing_button_label=View}, {hs_name=Rapid and consistent generation of functional microglia from reprogrammed hiPSCs to study neurodegeneration and neuroinflammation, hs_id=161961134491, hs_path=poster-rapid_and_consistent_generation_of_functional_ipsc_derived_microglia, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/bitbio-ioMicroglia_poster-V7-press_SfN%202022.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1504, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Emails/Header%20images/Header%20Images%20-%20ICC%20only/Microglia%20blog%20email%20header%20format.png, height=850}, year={label=2022, value=2022}, summary=<p>Raman, et al</p>
<p>bit.bio</p>
<p>2022</p>, date_published=1709251200000, sort_date=1669680000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioMicroglia, value=ioMicroglia}], media_contact=null, listing_button_label=View}, {hs_name=Modelling neurodegeneration: Human isogenic system to study FTD & ALS, hs_id=161961134492, hs_path=ftd-als-mapt-tdp-43-disease-models-adpd-2023-poster-mar23, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/bitbio_ioGlutamatergic%20Neurons%20MAPT%20TDP-43%20Disease%20Model%20poster_v2.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1966, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/MAPT%20N279K/N279K%20MAP2%20brightfield%20compressed%20rotated.png, height=1966}, year={label=2023, value=2023}, summary=<p>Oosterveen, et al</p>
<p>bit.bio & Charles River Laboratories</p>
<p>2023</p>, date_published=1709424000000, sort_date=1687478400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}], media_contact=null, listing_button_label=View}, {hs_name=Generation and characterisation of a panel of human iPSC-derived neurons and microglia carrying early and late onset relevant mutations for Alzheimer’s disease, hs_id=161961134495, hs_path=slas2024-ad-poster, button_label=null, button_link=null, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1800, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/Microglia%20hero%202%20resize%20brighten%20v2%20compress.png, height=1800}, year={label=2024, value=2024}, summary=Smith, et al. <br>bit.bio<br>2024, date_published=1711065600000, sort_date=1708300800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}], media_contact=Smith et al. <br>bit.bio<br>2024, listing_button_label=Download}, {hs_name=Lipid-Bilayer-Supported 3D Printing of Human Cerebral Cortex Cells Reveals Developmental Interactions , hs_id=161968263464, hs_path=lipid-bilayer-supported-3d-printing-of-human-cerebral-cortex-cells-reveals-developmental-interactions, button_label=Read more, button_link=https://onlinelibrary.wiley.com/doi/10.1002/adma.202002183, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=666, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources/Resource-thumbs/resized/New%20Project%20(13).jpeg, height=428}, year={label=2020, value=2020}, summary=<p>Zhou L, et al </p>
<p><em>Advanced Science News</em></p>
<p>2020</p>
<p><a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">Using ioGlutamatergic Neurons</a></p>, date_published=1592092800000, sort_date=1598659200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Zhou L, et al</p>
<p><em>Advanced Science News</em></p>
<p>2020</p>
<p>Using <a href="https://www.bit.bio/glutamatergic-neurons" rel="noopener">ioGlutamatergic Neurons</a></p>, listing_button_label=Read more}, {hs_name=Reprogramming the stem cell for a new generation of cures, hs_id=161968263465, hs_path=reprogramming-the-stem-cell-for-a-new-generation-of-cures, button_label=Read more, button_link=https://www.ddw-online.com/reprogramming-the-stem-cell-for-a-new-generation-of-cures-1459-202004/, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=2092, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/bit.bio%20ioGlutamatergic%20Neurons%202022.jpg, height=1584}, year={label=2020, value=2020}, summary=<p>Davenport A, Frolov T & Kotter M</p>
<p><em>Drug Discovery World</em></p>
<p>2020</p>
<p> </p>
<p> </p>, date_published=1585872000000, sort_date=1597968000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=ioMotor Neurons, value=ioMotor Neurons}, {label=ioAstrocytes, value=ioAstrocytes}], media_contact=<p><span>Davenport A, Frolov T & Kotter M.</span></p>
<p><em>Drug Discovery World</em></p>
<p>2020</p>
<p> </p>
<p> </p>, listing_button_label=Read more}, {hs_name=Inducible and Deterministic Forward Programming of Human Pluripotent Stem Cells into Neurons, Skeletal Myocytes, and Oligodendrocytes , hs_id=161968263466, hs_path=inducible-and-deterministic-forward-programming-of-human-pluripotent-stem-cells, button_label=Read more, button_link=https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(17)30083-8, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=759, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/publication-Inducible-and-deterministic-colour.jpg, height=461}, year={label=2017, value=2017}, summary=<p>Pawlowski M et al<br><em>Stem Cell Reports</em></p>
<p>2017</p>, date_published=1491868800000, sort_date=1499385600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p><span>Pawlowski M, et al</span></p>
<p><em>Stem Cell Reports</em></p>
<p>2017</p>
<p> </p>, listing_button_label=Read more}, {hs_name=Compounds co-targeting kinases in axon regulatory pathways promote regeneration and behavioral recovery after spinal cord injury in mice, hs_id=161968263467, hs_path=compounds-co-targeting-kinases-in-axon-regulatory-pathways-promote-regeneration-and-behavioral-recovery-after-spinal-cord-injury-in-mice, button_label=Read more, button_link=https://pubmed.ncbi.nlm.nih.gov/35588791/, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=1871, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Publications/bit.bio_single%20image_ioGlutamatergic%20Neurons%20Mah%20et%20al%202022.jpg, height=1417}, year={label=2022, value=2022}, summary=<p>Mah, et al</p>
<p><em>Experimental Neurology</em></p>
<p>2022</p>
<p>Using <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">ioGlutamatergic Neurons</a></p>, date_published=1652659200000, sort_date=1667260800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Mah, et al</p>
<p><em>Experimental Neurology</em></p>
<p>2022</p>
<p>Using <a href="https://www.bit.bio/glutamatergic-neurons" rel="noopener">ioGlutamatergic Neurons</a></p>, listing_button_label=Read more}, {hs_name=Glutamatergic Neurons and Brain Cyst Formation , hs_id=161968263468, hs_path=glutamatergic-neurons-and-brain-cyst-formation, button_label=Read more, button_link=https://www.frontiersin.org/articles/10.3389/fcimb.2021.788303/full, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=2092, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/bit.bio%20ioGlutamatergic%20Neurons%202022.jpg, height=1584}, year={label=2022, value=2022}, summary=<p>Bando, et al</p>
<p><em>Frontiers in Cellular and Infection Microbiology</em></p>
<p>2022</p>
<p>Using<a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank"> ioGlutamatergic Neurons</a></p>
<p> </p>
<p> </p>, date_published=1641859200000, sort_date=1667347200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Bando, et al<br><br><em>Frontiers in Cellular and Infection Microbiology</em></p>
<p>2022</p>
<p>Using <a href="https://www.bit.bio/glutamatergic-neurons" rel="noopener">ioGlutamatergic Neurons</a></p>, listing_button_label=Read more}, {hs_name=Interferon-γ exposure of human iPSC-derived neurons alters major histocompatibility complex I and synapsin protein expression , hs_id=161968263469, hs_path=interferon-γ-exposure-of-human-ipsc-derived-neurons-alters-major-histocompatibility-complex-i-and-synapsin-protein-expression, button_label=Read more, button_link=https://www.frontiersin.org/articles/10.3389/fpsyt.2022.836217/full, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=3742, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Publications/bit.bio%20ioGlutamatergic%20Neurons%20pavlinek%20et%20al%202022.jpg, height=2836}, year={label=2022, value=2022}, summary=<p>Pavinlek, et al</p>
<p><em>Frontiers in Psychiatry</em></p>
<p>2022</p>
<p>Using <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">ioGlutamatergic Neurons</a></p>
<p> </p>
<p> </p>, date_published=1663113600000, sort_date=1667433600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Pavinlek, et al<br><br><em>Frontiers in Psychiatry</em></p>
<p>2022</p>
<p>Using <a href="https://www.bit.bio/glutamatergic-neurons" rel="noopener">ioGlutamatergic Neurons</a></p>, listing_button_label=Read more}, {hs_name=Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication, hs_id=178039124124, hs_path=rewiring-of-the-promoter-nature-article, button_label=Read more, button_link=https://www.nature.com/articles/s41467-023-41919-x, type={label=Publication, value=Publication}, thumbnail={alt_text=bitbio-ioGlutamatergic-Neurons, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2023, value=2023}, summary=<p>Chakraborty et al<br><em>Nature Communications</em><br>2023</p>
<p>Featuring <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">ioGlutamatergic Neurons</a></p>, date_published=1697155200000, sort_date=1697155200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Chakraborty et al<br><em>Nature Communications</em><br>2023</p>
<p>Featuring <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">ioGlutamatergic Neurons</a></p>, listing_button_label=Read more}, {hs_name=HNRNPH1 regulates the neuroprotective cold‐shock protein RBM3 expression through poison exon exclusion, hs_id=178039124146, hs_path=hnrnph1-regulates-the-neuroprotective-cold-shock-protein-rbm3-expression-through-poison-exon-exclusion-article, button_label=Read more, button_link=https://www.embopress.org/doi/full/10.15252/embj.2022113168, type={label=Publication, value=Publication}, thumbnail={alt_text=bitbio-ioGlutamatergic-Neurons, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2023, value=2023}, summary=<p>Qiaojin Lin et al</p>
<p><em>The EMBO Journal</em></p>
<p>2023</p>
<p>Featuring opti-ox powered hiPSC-derived <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">glutamatergic neurons</a> with constitutive expression of Cas9</p>, date_published=1685404800000, sort_date=1685404800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p>Qiaojin Lin et al</p>
<p><em>The EMBO Journal</em></p>
<p>2023</p>
<p>Featuring opti-ox powered hiPSC-derived <a href="https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001" rel="noopener" target="_blank">glutamatergic neurons</a> with constitutive expression of Cas9</p>, listing_button_label=Read more}, {hs_name=Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons, hs_id=178046423390, hs_path=schizophrenia-risk-gene-znf804a-controls-ribosome-localization-and-synaptogenesis-in-developing-human-neurons-article, button_label=Read more, button_link=https://www.biorxiv.org/content/10.1101/2024.02.02.578424v1, type={label=Publication, value=Publication}, thumbnail={alt_text=bitbio-ioGlutamatergic-Neurons, width=1700, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons/bitbio-ioGlutamatergic-Neurons.jpg, height=1300}, year={label=2024, value=2024}, summary=<p><span>Deepak P.</span><span> </span><span>Srivastava, et al</span></p>
<p><em>bioRxiv</em></p>
<p>2024</p>
<p>Featuring <a href="https://www.bit.bio/platform" rel="noopener" target="_blank">opti-ox </a>enabled glutamatergic neurons iPS cell line</p>, date_published=1706832000000, sort_date=1706832000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=<p><span>Deepak P.</span><span> </span><span>Srivastava, et al</span></p>
<p><em>bioRxiv</em></p>
<p>2024</p>
<p>Featuring <a href="https://www.bit.bio/platform" rel="noopener" target="_blank">opti-ox</a><sup> </sup>enabled glutamatergic neurons iPS cell line</p>, listing_button_label=Read more}, {hs_name=Circadian clocks in human cerebral organoids, hs_id=178051600304, hs_path=circadian-clocks-in-human-cerebral-organoids-article, button_label=Read more, button_link=https://www.biorxiv.org/content/10.1101/2024.02.20.580978v1.full, type={label=Publication, value=Publication}, thumbnail={alt_text=Hero image_CRISPR-Ready ioMicroglia, width=1422, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioMicroglia/Hero%20image_CRISPR-Ready%20ioMicroglia.png, height=1920}, year={label=2024, value=2024}, summary=<p>Rzechorzek, et al</p>
<p><em>bioRxiv</em></p>
<p>2024</p>
<p>Featuring <a href="https://www.bit.bio/platform" rel="noopener" target="_blank">opti-ox</a><sup> </sup>enabled microglia male iPS cell line and opti-ox enabled glutamatergic neurons iPS cell line</p>, date_published=1708473600000, sort_date=1708473600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioMicroglia, value=ioMicroglia}], media_contact=<p>Rzechorzek, et al</p>
<p><em>bioRxiv</em></p>
<p>2024</p>
<p>Featuring <a href="https://www.bit.bio/platform" rel="noopener" target="_blank">opti-ox</a><sup> </sup>enabled microglia male iPS cell line and opti-ox enabled glutamatergic neurons iPS cell line</p>, listing_button_label=Read more}, {hs_name=CRISPR and the Art of Perturbation Screening: Unbiased functional genomic screening meets the best human cellular models, hs_id=161968263475, hs_path=crispr-and-the-art-of-perturbation-screening-unbiased-functional-genomic-screening-meets-the-best-human-cellular-models, button_label=Explore ioCRISPR-Ready Cells, button_link=https://www.bit.bio/iocrispr-ready, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/CRISPR%20and%20the%20Art%20of%20Perturbation%20Screening-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Kam Dhaliwal <span>| </span>SVP Strategic Alliances | bit.bio</p>
<p><br>Talk at ELRIG CRISPR in Drug Discovery</p>, date_published=1706140800000, sort_date=1636070400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioSensory Neurons, value=ioSensory Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Consistent and scalable human iPSC-derived cells for in vitro disease modelling and drug discovery, hs_id=161968263476, hs_path=consistent-and-scalable-human-ipsc-derived-cells-for-in-vitro-disease-modelling-and-drug-discovery, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=900, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/DDD_banner_square.jpeg, height=652}, year={label=2020, value=2020}, summary=<p>Kam Dhaliwal <span>| </span> SVP Strategic Alliances | bit.bio<br>Dr Thomas Moreau <span>|</span> Head of Research | bit.bio</p>
<p><br>Talk at ELRIG Drug Discovery Digital</p>, date_published=1706400000000, sort_date=1600905600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}], media_contact=null, listing_button_label=Watch now}, {hs_name=Precision Cellular Reprogramming for Scalable and Consistent Human Neurodegenerative Disease Models, hs_id=161968263479, hs_path=precision-cellular-reprogramming-for-scalable-and-consistent-human-neurodegenerative-disease-models, button_label=Explore ioDisease Model Cells, button_link=https://www.bit.bio/iodisease-models, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Tech%20Nets%20online%20symposium%20header%20(1).png, height=1260}, year={label=2023, value=2023}, summary=<p>Madeleine Garrett | Field Application Specialist | bit.bio</p>, date_published=1709078400000, sort_date=1681344000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioMicroglia, value=ioMicroglia}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}, {label=ioMotor Neurons, value=ioMotor Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Industrialising Cellular Reprogramming: Leveraging opti-ox Technology to Manufacture Human Cells with Unprecedented Consistency, hs_id=161968263480, hs_path=industrialising-cellular-reprogramming-leveraging-opti-ox-technology-to-manufacture-human-cells-with-unprecedented-consistency, button_label=null, button_link=null, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1024, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Resources/video/Squarespace+Thumbnail+Template+-+AM23-01-01-01.png, height=768}, year={label=2023, value=2023}, summary=<p>Innovation showcase talk at ISSCR</p>
<p>Marius Wernig MD, PhD | Stanford </p>
<p>Mark Kotter, MD, PhD | bit.bio</p>, date_published=1709683200000, sort_date=1688083200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=CRISPR-Ready ioCells, value=CRISPR-Ready ioCells}], media_contact=<p>V1<br><br>2023<br><br>ISSCR 2023</p>, listing_button_label=Watch now}, {hs_name=ioGlutamatergic Neurons Wild Type and related disease models | User Manual, hs_id=161968263487, hs_path=glutamatergic-neurons, button_label=null, button_link=null, type={label=User manual, value=User manual}, thumbnail={alt_text=bit-bio ioGlutamatergic Neurons Day 14 Beta III Tubulin staining copy, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/ioGlutamatergic%20Neurons%20in%20Drug%20Discovery/bit-bio%20ioGlutamatergic%20Neurons%20Day%2014%20Beta%20III%20Tubulin%20staining%20copy.jpeg, height=1200}, year={label=2024, value=2024}, summary=<p>V11</p>
<p>bit.bio</p>
<p>2024</p>, date_published=1707955200000, sort_date=1713225600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}, {label=ioAstrocytes, value=ioAstrocytes}, {label=Product information, value=Product information}], media_contact=<p><span>V11</span></p>
<p><span>bit.bio</span></p>
<p><span>2024</span></p>, listing_button_label=Download}, {hs_name=Partnering with Charles River to advance CNS drug discovery with ioGlutamatergic Neurons, hs_id=161968263497, hs_path=in-conversation-with-charles-river-video-part-2, button_label=null, button_link=null, type={label=Video, value=Video}, thumbnail={alt_text=, width=687, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/CRL%20video%20posts/crl%202%20webpage%20image-2.png, height=476}, year={label=2021, value=2021}, summary=<p>Dr Marijn Vlaming | Head of Biology, et al.</p>
<p>Charles River & bit.bio</p>, date_published=1705017600000, sort_date=1637107200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch }, {hs_name=In Conversation with Charles River, hs_id=161968263498, hs_path=in-conversation-with-charles-river-video, button_label=Discover our ioCells portfolio, button_link=/discover-iocells, type={label=Video, value=Video}, thumbnail={alt_text=, width=834, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/CRL%20video%20posts/CRL%20video%20%231%20card%20for%20webpage%20(thinner%20gradient%20line)-1.png, height=590}, year={label=2021, value=2021}, summary=<p>Dr Marijn Vlaming <span>|</span> Head of Biology<br>Charles River</p>, date_published=1705363200000, sort_date=1636675200000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}], media_contact=null, listing_button_label=Watch}, {hs_name=Culturing Glutamatergic Neurons | How-to Video, hs_id=161966111374, hs_path=how-to-culture-ioglutamatergic-neurons, button_label=Download the user manual, button_link=/resources/ioglutamatergic-neurons-user-manual, type={label=Video tutorial, value=Video tutorial}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/How%20to%20prepare%20tissue%20culture%20vessels%20with%20PDL-Geltrex-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Dr Kaiser Karim <span>|</span> Scientist<br>bit.bio</p>, date_published=1705104000000, sort_date=1637020800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch now}, {hs_name=Preparing Culture Vessels for Glutamatergic Neurons | How-to Video, hs_id=161966111375, hs_path=how-to-prepare-tissue-culture-vessels-with-pdl-geltrex, button_label=Watch the next video, button_link=/resources/how-to-culture-ioglutamatergic-neurons, type={label=Video tutorial, value=Video tutorial}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/How%20to%20culture%20ioGlutamatergic%20Neurons-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Dr Kaiser Karim <span>|</span> Scientist<br>bit.bio</p>, date_published=1705190400000, sort_date=1636934400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}], media_contact=null, listing_button_label=Watch now}, {hs_name=Modelling neurodevelopment | Investigating the impact of maternal immune activation on neurodevelopment using human iPSC-derived cells, hs_id=161968263519, hs_path=modelling-neurodevelopment-webinar-2022, button_label=Explore ioGlutamatergic Neurons, button_link=https://www.bit.bio/products/nerve-cells/glutamatergic-neurons-wild-type-io1001, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/product-page/Colour%20webinar%20with%2060x%20Map2-com.jpg, height=732}, year={label=2022, value=2022}, summary=<p><span>Dr Deepak Srivastava | King’s College London<br><br></span><span></span></p>, date_published=1707436800000, sort_date=1649376000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Rethinking Developmental Biology With Cellular Reprogramming, hs_id=161968263524, hs_path=rethinking-developmental-biology-with-cellular-reprogramming, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Upcoming%20Webinars/Tech%20Nets%202023/bit.bio_ioGlutamatergic%20Neurons_20xMAP2(red)Hoescht(blue)_day12v2.png, height=1260}, year={label=2023, value=2023}, summary=<p>Mark Kotter | CEO and founder | bit.bio</p>
<p>Marius Wernig | Professor Departments of Pathology and Chemical and Systems Biology | Stanford University</p>, date_published=1709164800000, sort_date=1681776000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}, {label=Cell therapy, value=Cell therapy}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}], media_contact=null, listing_button_label=Watch now}, {hs_name=Addressing the Reproducibility Crisis | Driving Genome-Wide Consistency in Cellular Reprogramming, hs_id=161968263525, hs_path=addressing-the-reproducibility-crisis-driving-genome-wide-consistency-in-cellular-reprogramming, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1860, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Upcoming%20Webinars/Tech%20Nets%2019th%20July%202023/bit.bio_ioGlutamatergic%20Neurons_60xMAP2(red)Hoescht(blue)TUBB3(blue)_day4.jpg, height=1260}, year={label=2023, value=2023}, summary=<p>Dr Ania Wilczynska | Head of Computational Genomics | Non-Clinical | bit.bio</p>, date_published=1709769600000, sort_date=1689724800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=CRISPR-Ready ioGlutamatergic Neurons, value=CRISPR-Ready ioGlutamatergic Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Running Large-Scale CRISPR Screens in Human Neurons, hs_id=161968263528, hs_path=running-large-scale-crispr-screens-in-human-neurons, button_label=null, button_link=null, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1200, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Emails/Header%20images/Header%20Images%20-%20ICC%20only/Running%20Large-Scale%20CRISPR%20Screens%20in%20Human%20Neurons_email%20header.png, height=678}, year={label=2023, value=2023}, summary=<p>Emmanouil Metzakopian | Vice President, Research and Development | bit.bio</p>
<p>Javier Conde-Vancells | Director Product Management | bit.bio</p>, date_published=1710460800000, sort_date=1700524800000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=CRISPR-Ready ioCells, value=CRISPR-Ready ioCells}, {label=CRISPR-Ready ioGlutamatergic Neurons, value=CRISPR-Ready ioGlutamatergic Neurons}, {label=CRISPR-Ready ioMicroglia, value=CRISPR-Ready ioMicroglia}], media_contact=null, listing_button_label=Watch now}, {hs_name=Human iPSC-Based Models of Glial Cells for Studying Neurodegenerative Disease, hs_id=183096620633, hs_path=human-ipsc-based-models-of-glial-cells-for-studying-neurodegenerative-disease, button_label=null, button_link=null, type={label=Webinar, value=Webinar}, thumbnail={alt_text=bitbio-oligodendrocyte-like-cells-O4-MBP-immunocytochemistry, width=1000, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioOligodendrocyte-like%20cells/bitbio-oligodendrocyte-like-cells-O4-MBP-immunocytochemistry.jpg, height=1350}, year={label=2024, value=2024}, summary=Valentina Fossati, PhD | Senior Research Investigator | The New York Stem Cell Foundation<br><br>Inês Ferreira | Senior Product Manager | bit.bio, date_published=1732233600000, sort_date=1732233600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioOligodendrocyte-like cells, value=ioOligodendrocyte-like cells}], media_contact=null, listing_button_label=Watch now}])
Hoescht(blue)TUBB3(blue)_day4.jpg?width=604&name=bit.bio_ioGlutamatergic%20Neurons_60xMAP2(red)Hoescht(blue)TUBB3(blue)_day4.jpg)
Human iPSC-Based Models of Glial Cells for Studying Neurodegenerative Disease
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Producing 3D Neuronal Microtissues for Preclinical Drug Screening using ioGlutamatergic Neurons
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Sartorius application note - Advanced in vitro Modeling of Human iPSC-derived Neuronal Mono- and Co-cultures with Microglia
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ioGlutamatergic Neurons
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ioGlutamatergic Neurons Wild Type and related disease models | User Manual
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Circadian clocks in human cerebral organoids
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Generation and characterisation of a panel of human iPSC-derived neurons and microglia carrying early and late onset relevant mutations for Alzheimer’s disease
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Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons
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Generating publishable neuroscience research in 12 weeks with ioGlutamatergic Neurons
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Running Large-Scale CRISPR Screens in Human Neurons
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Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication
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Addressing the Reproducibility Crisis | Driving Genome-Wide Consistency in Cellular Reprogramming
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Cell culture hacks | human iPSC-derived glutamatergic neurons
Read this blog on glutamatergic neuron cell culture for our top tips on careful handling, cell plating and media changes to achieve success from the outset.
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CRISPR-Ready ioCells
CRISPR-Ready ioGlutamatergic Neurons
cat no | io1090
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ioDisease Model Cells
ioGlutamatergic Neurons HTT 50CAG/WT
cat no | ioEA1004
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT N279K/WT
cat no | io1009
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT P301S/WT
cat no | io1015
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ioDisease Model Cells
ioGlutamatergic Neurons TDP-43 M337V/WT
cat no | ioEA1006
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ioDisease Model Cells
ioGlutamatergic Neurons PRKN R275W/WT
cat no | io1013
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ioDisease Model Cells
ioGlutamatergic Neurons GBA null/R159W
cat no | io1007
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ioDisease Model Cells
ioGlutamatergic Neurons APP KM670/671NL/WT
cat no | io1061
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ioDisease Model Cells
ioGlutamatergic Neurons APP V717I/WT
cat no | io1067
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ioDisease Model Cells
ioGlutamatergic Neurons PSEN1 M146L/WT
cat no | io1072
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ioDisease Model Cells
ioGlutamatergic Neurons PINK1 Q456X/WT
cat no | io1079
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ioDisease Model Cells
ioGlutamatergic Neurons SNCA A53T/WT
cat no | io6005
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT N279K/N279K
cat no | io1014
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ioDisease Model Cells
ioGlutamatergic Neurons MAPT P301S/P301S
cat no | io1008
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ioDisease Model Cells
ioGlutamatergic Neurons TDP-43 M337V/M337V
cat no | ioEA1005
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ioDisease Model Cells
ioGlutamatergic Neurons PRKN R275W/R275W
cat no | io1020
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ioDisease Model Cells
ioGlutamatergic Neurons APP KM670/671NL / KM670/671NL
cat no | io1059
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ioDisease Model Cells
ioGlutamatergic Neurons APP V717I/V717I
cat no | io1063
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ioDisease Model Cells
ioGlutamatergic Neurons PSEN1 M146L/M146L
cat no | io1069
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ioDisease Model Cells
ioGlutamatergic Neurons PINK1 Q456X/Q456X
cat no | io1076
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ioDisease Model Cells
ioGlutamatergic Neurons SNCA A53T/A53T
cat no | io1088
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What scientists say about ioGlutamatergic Neurons
Dr Shushant Jain
Group Leader | In Vitro Biology | Charles River, 2021
"These cells enable us to move rapidly as from the moment of plating within 4-7 days we have mature and functional neurons."
Dr Mariangela Iovino
Senior Group Leader | Biology Discovery | Charles River
“Our major surprise when we first used the ioGlutamatergic Neurons was that after thawing the cells in 384-well format, we could see immediately after 2 days a nice neuronal network, and there was no well to well variability within the same plate. This made our assay quite robust.”
Dr Koby Baranes
Research Associate | University of Cambridge
"These cells provide a reliable and pure source of glutamatergic neurons, resembling primary human ones. They are ready-to-use which makes it much more easy for tissue culture work and for reproducible results.”
Dr Jeremy Anton
Scientist | Charles River
"ioGlutamatergic Neurons are easy to use with a simple application protocol. They recover quickly after thaw and are able to form a mature mesh of neurons ideal for testing within a few days."
Professor Deepak Srivastava
Professor | Molecular Neuroscience | King’s College London and Group Leader | MRC Centre for Developmental Disorders
"ioGlutamatergic Neurons provide a useful system for studying the effects of IFN-gamma in a pure population of glutamatergic cells"
Wild type and isogenic disease model cells: A true comparison
Be confident in your data by pairing ioDisease Model cells with the genetically matched ioWild Type control
